Red box shows differentially methylated peaks upstream of the gene.
Black arrow shows the transcriptional direction of the gene. IGV image created from MeDIP-seq data for selenbp1 gene. Heatmap of RPM values of DNA methylation at promoter regions of anti-ROS genes. An asterisk represents significant difference of ROS level compared to 28☌ sample (P<0.05). Error bars represent standard deviations (SD) (n = 3). The DCF positive cells were measured by flow cytometric analysis. ZF4 cells were treated with cold stress at 18☌ for 3 h, 6 h, 12 h, 1 d, 3 d and 5 d. Cold stress induced ROS production in ZF4 cells. **: P<0.01, ***: P<0.001.Īnti-ROS genes were hypomethylated under cold pressure.Ī. An asterisk represents significant difference compared to 28☌ sample (P<0.05). The student t test was used on measurements from 18☌ /5d, 18☌ /30d and 28☌ samples from 3 experimental replicates. Statistical analysis was performed using GraphPad Prism 5 software. Relative quantitation was carried out by the comparative threshold cycle (CT) method. RT-qPCR analysis of p53, atp5j, epb4 and trpm4c. Frequency of DNA methylation was calculated by the comparative threshold cycle (CT) method. MeDIP-qPCR analysis of p53, atp5j, epb4 and trpm4c. Red boxes show differentially methylated peaks upstream of genes. Black arrows show the transcriptional direction of genes. IGV images created from MeDIP-seq data for p53, atp5j, epb4 and trpm4c gene loci. P values for enrichment of GO categories are shown in the figure.ĭNA methylation in promoter regions of cold-related genes and expression analysis.Ī. = 1.1 or = 1.1 or = 1.5 and P value < = 0.05 are shown in the figure.
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